Skip to main content
Fig. 4 | Molecular Brain

Fig. 4

From: Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system

Fig. 4

Targeting long-range GABAergic neurons using a DLX6-CCre-IRES-eGFP mouse and virus. a Schematic of stereotaxic injection of lentiviral particles to observe recombination of Cre recombinase in DLX6-CCre-IRES-eGFP mice. The mTagBFP2-only lentiviral particles were injected into one hemisphere or the striatum, while the NCre-IntN-carrying lentiviral particles were injected into the other hemisphere. b In the hemisphere injected with the mTagBFP2-only lentiviral particles, no TdTomato signal was observed. However, in the hemisphere injected with NCre-IntN-carrying lentiviral particles, a TdTomato signal was observed, indicating the presence of functional Cre recombinase. Scale bar, 25 μm. c Venn diagram showing the percentage of cells expressing TdTomato. d Microscopic image showing the injection site of CTb-555 in the DS of wildtype mice. Scale bar, 200 μm. e CTb-555-labeled cells were observed in both the CeA and BLA. However, immunohistochemical analysis illustrated that only labeled cells in the CeA were GABAergic, while those in the BLA were not. Scale bars, 200 μm (10x) and 20 μm (40x). f Schematic diagram showing projection from CeA to DS. The FuG-E-NCre virus was injected into the DS of Dlx6-CCre-IRES-eGFP::LoxP-stop-LoxP-TdTomato mice, which was retrogradely transferred to the CeA. Cre recombination is expected to occur in GABAergic neurons in the CeA that project to the DS. g When FuG-E-NCre pseudotyped lentiviral particles were injected into the Dlx6-CCre-IRES-eGFP::LoxP-stop-LoxP-TdTomato mice, TdTomato cells were observed in the CeA. Scale bars, 200 μm (10x) and 20 μm (40x). h Venn diagram showing the percentage of cells expressing TdTomato in the CeA

Back to article page