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Table 2 Primer sequences for real-time qPCR analysis

From: Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum

mRNA Oligonucleotide Sequence (5′-3′) Amplicona (bp) Gene Bank No.b
IL-1β F: TCC AGG ATG AGG ACA TGA GCA C
R: GAA CGT CAC ACA CCA GCA GGT TA
105 NM_008361.3
IL-6 F: CCA CTT CAC AAG TCG GAG GCT TA
R: CCA GTT TGG TAG CAT CCA TCA TTT C
169 NM_031168.1
TNF-α F: CTT CTG TCT ACT GAA CTT CGG
R: CAG GCT TGT CAC TCG AAT TTT
134 NM_013693.3
CD45 F: TGG AAT GAC CTC AAG GTG TCC TC
R: GCT GTA CAC ACC CAC AGC ACT CTT
108 NM_001111316.2
MMP-13 F: TTC TTG TTG AGC TGG ACT CCC TGT
R: TGC TCT GCA AAC ACA AGG TCT TCC
98 NM_008607.2
GADPH F: GCC TTC CGT GTT CCT ACC
R: CCT CAG TGT AGC CCA AGA TG
142 NM_001289726.1
  1. aPredicted number size of PCR product; bNCBI reference sequence