Skip to main content
Fig. 1 | Molecular Brain

Fig. 1

From: Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis

Fig. 1

Brain morphological alteration of the En1-Cre-driven Yy1 knockout mice. a, b Immunostaining with antibody against YY1 showing the complete inactivation of YY1 throughout the Yy1 knockout mutant MHB neuroepithelium at E9.5. White arrowheads and asterisks pointing to the meninges and roof plate cells that still express YY1, respectively. c-e Gross morphology of no Cre control littermate (c), heterozygous (d), and homozygous mutant (e) at P0. The En1Cre/+YY1fx/+ mice showed no observable difference compared to the no Cre control littermates. Yellow arrowhead pointing to the truncated midbrain and missing cerebellum in En1Cre/+; YY1fx/fx mutant. The red arrow pointing to the 4th ventricle choroid plexus. The figures were at the same magnification. f, g Histology of P0 control and mutant brain. h-m H&E staining of embryonic control and mutant littermates MHB region at E9.5, E10.5 and E11.5. Neuroepithelial tissue-loss could be observed in mutant at E11.5 (red arrowheads). Cb, cerebellum; CP, choroid plexus; IC, inferior colliculus; SC, superior colliculus; vMH, ventral mid-hindbrain

Back to article page