Fig. 3

Inactivation of Yy1 changed cell cleavage plane and decreased cell polarity proteins of MHB NECs. a Immunostaining of β-catenin and γ-tubulin in E10.5 MHB neuroepithelium showing loss-of-Yy1 resulted in reduced β-catenin level and altered centrosome orientation. b Representative figure showing the measuring method of the mitotic spindle orientation angle. cYy1-ablated MHB NECs showed significant reduction in the mitotic spindle orientation angle. (the angle in the controls equals to 68.52 ± 2.544, n = 49, while in mutants is 54.58 ± 3.544, n = 50, **, p = 0.0019). d Mutant NECs showed significant increase in the percentage of cells undergoing asymmetric division. e Western blotting showing β-catenin level was reduced in Yy1 KO mes/r1 region. f-k Immunostaining of cell polarity proteins β-catenin, ZO1 and E-cadherin showing at E11, deletion of Yy1 in MHB NECs resulted in the partially destructed tissue integrity. Arrowheads showing reduced expression of the markers throughout the mes/r1 neuroepithelium