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Fig. 5 | Molecular Brain

Fig. 5

From: Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis

Fig. 5

Inactivation of Yy1 in mouse MHB reduced the expression of Wnt1 by transcriptional deactivation. a Real-time qPCR results showing decreased expression of Wnt1 but no other Wnt ligands in the Yy1-inactivated mes/r1 region at E9.5. N numbers for each gene tested are shown in the bars of controls. Unpaired t-test. ***, p < 0.001. b-d Whole-mount in situ hybridization using Wnt1-specific RNA probe showing reduced expression of Wnt1 in mutant MHB region at E10.5. e, f Schematic illustration of Wnt1 luciferase promoter analysis, with (f) showing the luciferase reporter assay results. Unpaired t-test, n = 6. For mutated binding sites, n = 4. *, p = 0.036; **, p < 0.01; ***, p < 0.001. g Representative results of gel electroporation using ChIP-qPCR products. h Fold enrichment of YY1-putative binding-site fragments immunoprecipitated with YY1. ChIP-qPCR results suggest YY1 binds to Wnt1 promoter binding sites in E10.5 mouse mes/r1 cells. Each p-value is shown under the binding-site group number. Unpaired t-test, N = 4

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