Table 2 Primers for real-time qPCR
target gene name | primer sequence | |
|---|---|---|
βActin-qPCR | F | ACGCGCAGCCACTGT |
R | CTGACCCATTCCCACCATCA | |
bax-qPCR | F | TGCTAGCAAACTGGTGCTCA |
R | GCCTTCCCAGCCACCCT | |
Bcl2-qPCR | F | CAACATCGCCCTGTGGATGA |
R | AGGGTCTTCAGAGACAGCCA | |
Ccna1-qPCR | F | CTGACCGTTCCAACCACCAA |
R | TGCAGCAACCAAGGAAGGAA | |
Ccna2-qPCR | F | TTACCCGGAGCAAGAAAACC |
R | ACGTTCACTGGCTTGTCTTCTAA | |
Ccnb1-qPCR | F | AGTGACGTAGACGCAGATGAT |
R | GGTCTCCTGAAGCAGCCTAAA | |
Ccnd1-qPCR | F | GCGTACCCTGACACCAATCTC |
R | CTCCTCTTCGCACTTCTGCTC | |
Ccnd2-qPCR | F | GTACCCGCCGTCGATGATT |
R | CAGCAGCAGAGCTTCGATTT | |
Ccne1-qPCR | F | AGCACTTTCTGCAGCGTCAT |
R | TCAAAGAAGTCCTGTGCCAAGT | |
cdc6-qPCR | F | TCAGTCCCCGAAAACGTCTG |
R | TTCCACGTATGTGAGCGAGG | |
cdk1-qPCR | F | TGCAGGACTCCAGGCTGTAT |
R | AGGCCGAAATCAGCCAGTTT | |
cdk2-qPCR | F | GAGTCCCTGTCCGAACTTACA |
R | TCCTTGTGATGCAGCCACTTCTA | |
cdk4-qPCR | F | ATGTGGAGCGTTGGCTGTAT |
R | GGGCTCGGAAGGCAGAGATT | |
cdk6-qPCR | F | GCGTACCCACAGAAACCATAAAG |
R | CCGAGGTAAGGGCCATCTGAAA | |
cdkn1a-qPCR | F | CCTGGTGATGTCCGACCTG |
R | CCATGAGCGCATCGCAATC | |
Cend1-qPCR | F | CTCCTGAGCACTCCTCGGTAT |
R | GGTTTGGGGCTTGTGTGACT | |
Gbx2-qPCR | F | AGACGGCAAAGCCTTCTTG |
R | TCGGGTCATCTTCCACCTTT | |
Mdm2-qPCR | F | CCAGGCCAATGTGCAATACCAACA |
R | TGCGCTCCAACGGACTTTAACAAC | |
Otx2-qPCR | F | ATCTCCCTGAGAGCGGAACC |
R | CAGGGTCCTTGGTGGGTAGA | |
Pax5-qPCR | F | ATTACCCGACTCCTCGGACCAT |
R | TGATGGGCAAGTTCCACTATCC | |
Trp53-qPCR | F | ATCCTGGCTGTAGGTAGCGA |
R | ATCCGACTGTGACTCCTCCA | |
Wnt1-qPCR | F | GGTTTCTACTACGTTGCTACTGG |
R | GGAATCCGTCAACAGGTTCGT | |
Wnt3a-qPCR | F | CTCCTCTCGGATACCTCTTAGTG |
R | GCATGATCTCCACGTAGTTCCTG | |
Wnt5a-qPCR | F | CAACTGGCAGGACTTTCTCAA |
R | CATCTCCGATGCCGGAACT | |
Yy1-qPCR | F | GCAGAGTGTGGCAAAGCGTT |
R | CTGAGCAAACTTCTTATTACAACCG | |
Yy2-qPCR | F | TTGATGCCTGCAACAAGAAGT |
R | AGGCTTCAAAGGGACTCTCACT | |
Wnt1pro-bs2 | F | CGGAGTCGCTGGCTAGAAC |
R | GCTGTCCTCTCGAAGTCCGT | |
Wnt1pro-bs3 | F | TAGCCCACAGAGGCAAACTG |
R | CACTTCCCTCACCCAGGAAC | |
Wnt1pro-NC1 | F | CCTCCCTTCCTTGTCCAACC |
R | CAATGCCTTTCGGGTCCTCT | |
Wnt1pro-NC2 | F | TTCACTCCTGGGACCTCGAT |
R | GAGGAGGCTTTGGGAGACAC | |