Fig. 3

α-tubulin deacetylation by HDAC6 is not related with Golgi polarization. Neurons expressing α-TAT1, α-tubulin acetyl transferase 1, did not change dendrite growth. a HDAC6 knockdown neurons affected dendrite growth. αTAT1 overexpressing neurons had normal morphology. Scale bar, 50 μm. b Sholl graphs of neurons expressing Venus or HDAC6 shRNA or αTAT1. Data represents average of three independent experiments (n = 10 for each group in each experiment). c-e Graphs represent the number of primary dendrites (c), longest dendrite length (d), and total dendrite length (e). f The Golgi apparatus was stained by GM130 antibody (red) in neurons. Unlike HDAC6 knockdown neurons, αTAT1 overexpression neurons did not affect Golgi polarization. Scale bar, 50 μm. g Quantification of the percentage of neurons with dendritic Golgi. Data represents average of three independent experiments (n = 80–120 for each group in each experiment). h-j Neurons expressing tubulin acetylation mutants did not influence dendrite development and Golgi polarization. h Expression of deacetylation mimetic form of tubulin did not rescue HDAC6 knockdown phenotype. Overexpression of acetylation mimetic form of tubulin did not affect dendrite development and Golgi deployment in primary hippocampal neurons. The Golgi apparatus was stained by GM130 (red). Scale bar, 50 μm. i, j Quantification of the number of primary dendrites (i) and percentage of neurons with dendritic Golgi (j). Data represents average of three independent experiments (n = 60–120 for each group in each experiment). *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed Student’s t-test. The symbol # represents the number. Error bar means ± S.E.M. in all graphs