Skip to main content
Fig. 1 | Molecular Brain

Fig. 1

From: Alleviating toxic α-Synuclein accumulation by membrane depolarization: evidence from an in vitro model of Parkinson’s disease

Fig. 1

Preformed fibrils (PFFs) treatment induced α-Syn inclusion accumulations in the differentiated SH-SY5Y cells. a1 and b1 Representative IR-DIC image of differentiated SH-SY5Y neuroblastoma cells treated with (B1) or without (A1) 25 μg/ml PFFs treatment for 3 days. Note differentiated cells show long neurites and complex network connections. a2 and b2 Representative whole-cell voltage responses to 500 ms rectangular current injections of 0, 50, 120pA under the current clamp mode in differentiated SH-SY5Y cells treated with (B2) or without (A2) 25 μg/ml PFFs for 3 days. Arrow points to the action potentials generated. c Summary of the mean resting membrane potentials in control and 25 μg/ml PFFs-treated SH-SY5Y cells. Error bars indicate SEM. d Summary of the mean membrane potential threshold in control and 25 μg/ml PFFs-treated SH-SY5Y cells. Error bars indicate SEM. Two-tailed unpaired t test, *p < 0.05. e Immunofluorescent staining of α-Syn (green) in the differentiated SH-SY5Y cells following the treatment of 0, 5, 10, 25 μg/ml PFFs for 3 days. Scale bar: 200 μm. f Summary of the mean integrated intensity per cell of α-Syn staining in the differentiated SH-SY5Y cells following the treatment of 0, 5, 10, 25 μg/ml PFFs for 3 days. Error bars indicate SEM. One-way ANOVA, Tukey’s HSD post-hoc test. **p < 0.01. ***p < 0.001

Back to article page