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Fig. 3 | Molecular Brain

Fig. 3

From: Alleviating toxic α-Synuclein accumulation by membrane depolarization: evidence from an in vitro model of Parkinson’s disease

Fig. 3

Membrane depolarization induced by KCL and Picrotoxin reduces α-Syn aggregation in PFF-treated SH-SY5Y cells. a Immunofluorescent staining of α-Syn (green) in the differentiated SH-SY5Y cells following the treatment of control, PFF only, KCL then PFF, Picrotoxin then PFF, TTX then PFF, PFF + KCL, PFF + Picrotoxin, or PFF + TTX for 3 days. Scale bar: 200 μm. b Summary of the mean integrated intensity per cell of α-Syn staining in the differentiated SH-SY5Y cells following the treatment of PFF only, KCL then PFF, Picrotoxin then PFF, TTX then PFF, PFF + KCL, PFF + Picrotoxin, or PFF + TTX for 3 days. Error bars indicate SEM. One-way ANOVA, Tukey’s HSD post-hoc test. ***p < 0.001. c Average percentage changes expressed as percentage of the PFF only group in the integrated intensity per cell of α-Syn staining in the differentiated SH-SY5Y cells following the treatment of KCL then PFF, Picrotoxin then PFF, TTX then PFF, PFF + KCL, PFF + Picrotoxin, or PFF + TTX for 3 days. Error bars indicate SEM. One-way ANOVA, Tukey’s HSD post-hoc test. ***p < 0.001 compared with the PFF only group

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