Fig. 5

Effect of maternal zinc supplementation on cortico-striatal AMPAR-mediated EPSCs in adult Shank3^−/− offspring mice. MSN striatal evoked AMPAR-mediated EPSC amplitudes measured in adult wildtype (WT) and Shank3^−/− offspring mice from normal and supplemented maternal zinc diet groups. a Left: In comparison to WT, adult Shank3^−/− mice from the control or supplemented maternal zinc diet (MZD) group displayed significantly decreased evoked AMPAR-mediated ESPC amplitudes. Right: Frequency histogram of AMPAR-mediated EPSC amplitudes illustrating a shift towards smaller AMPAR-mediated EPSC amplitudes in Shank3^−/− mice from mothers on the control (top) or supplemented (bottom) zinc diet. WT control MZD n = 16 neurons from 7 mice, Shank3^−/− control MZD n = 13 neurons from 7 mice, WT supplemented MZD n = 21 neurons from 10 mice, Shank3^−/− supplemented MZD n = 14 neurons from 5 mice. b Representative AMPAR-mediated EPSC traces from adult WT and Shank3^−/− mice from mothers that were fed with the control zinc diet (left), and WT and Shank3^−/− mice from mothers that were fed with the supplemented zinc diet (right). c Adult Shank3^−/− mice from the control maternal zinc diet (MZD) have a significantly reduced paired-pulse ratio (PPR) in comparison to wildtype (WT) mice. No significant difference was observed in PPR between Shank3-wildtype and Shank3^−/− mice from mothers that were fed with the supplemented zinc diet. WT control MZD n = 7 neurons from 3 mice, Shank3^−/− control MZD n = 12 neurons from 5 mice, WT supplemented MZD n = 9 neurons from 3 mice, Shank3^−/− supplemented MZD n = 11 neurons from 4 mice. d Representative traces of PPR from wildtype (top left) and Shank3^−/− mice (top right) from mothers that were fed with the control zinc diet, and wildtype (bottom left) and Shank3^−/− mice (bottom right) from mothers that were fed with the supplemented zinc diet. All values are presented as mean ± standard error of the mean. Individual data points represent individual neurons, and were statistically analysed using two-way ANOVA with Tukey’s multiple comparisons test. NS = not significant, *p < 0.05, *** p < 0.001