Fig. 6
Effect of maternal zinc supplementation on cortico-striatal NMDAR-mediated EPSCs in adult Shank3−/− mice. Adult MSN NMDAR-mediated EPSC amplitudes measured in adult wildtype (WT) and Shank3−/− offspring mice from normal and supplemented maternal zinc diet groups. a Evoked NMDAR-mediated EPSC amplitudes are significantly reduced in adult Shank3−/− offspring mice from mothers that were fed with the control zinc diet in comparison to adult wildtype mice from mothers that were fed with the control zinc diet. The supplemented maternal zinc diet also induced a significant decrease in adult WT mice. NMDAR-mediated EPSC amplitudes were comparable between WT mice from mothers that were fed with the control zinc diet and Shank3−/− mice from mothers that were fed with the supplemented zinc diet. b NMDAR decay kinetics, measured as weighted tie constant tau (tw), were not significantly different between adult WT and Shank3−/− mice from mothers that were fed with the control or supplemented zinc diet. c Representative NMDAR-mediated EPSC traces from adult WT and Shank3−/− mice from mothers that were fed with the control zinc diet (left), and WT and Shank3−/− mice from mothers that were fed with the supplemented zinc diet (right). WT control MZD n = 9 neurons from 5 mice, Shank3−/− control MZD n = 7 neurons from 5 mice, WT supplemented MZD n = 7 neurons from 3 mice, Shank3−/− supplemented MZD n = 7 neurons from 4 mice. All values are presented as mean ± standard error of the mean. Individual data points represent individual neurons, and were statistically analysed using two-way ANOVA with Tukey’s multiple comparisons test. NS = not significant, *p < 0.05