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Fig. 4 | Molecular Brain

Fig. 4

From: TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion

Fig. 4

Effects of TMEM175 overexpression on neuronal death after OGD/R. a Transfection efficiency of TMEM175 overexpression plasmid in primary neurons (OGD/R vs. control, **p = 0.0054; OGD/R + Vector vs. OGD/R + Over-TMEM175, ***p = 0.0004, control vs. control +Over-TMEM175, ***p = 0.0008). b Hoechst staining. The arrow points to dead neurons showing nuclear condensation. c Quantitative analysis of cell death via Hoechst staining. Data are presented as the percentage of the dead neurons showing nuclear condensation. (OGD/R vs. control, ****p < 0.0001; Vector vs. Over-TMEM175, ****p < 0.0001). d The live/dead cell viability assay. Healthy neurons appear green and the condensed nuclei of dead neurons appear red. e Quantitative analysis of cell death via the cell viability live/dead assay. Data are presented as the ratio of dead neurons to live neurons. (OGD/R vs. control, ****p < 0.0001; Vector vs. Over-TMEM175, ***p = 0.0009)

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Molecular Brain

ISSN: 1756-6606