Fig. 6

RNA sequencing reveals downregulated inflammatory transcriptional landscape in miR-22-deficient mice. a Volcano plot based on RNA sequencing, shows that miR-22 deficiency in mice resulted in the decreased expression of 18 immune-associated genes (labelled with names) in the hippocampus 24 h after status epilepticus. b Bar plot shows the numbers of genes in different classes of 437 transcription factors identified for the 18 immune-associated genes, including 93 transcription repressors and co-repressors (21.3%). c Bar plot showing the numbers of the immune-associated genes targeted by each of the 25 miR-22 mediated transcriptional repressors. Each of these repressors regulates several the immune-associated targets. d Volcano plot showing gene expression, magnitude of changes and statistical significance of differential expression analysis of the 25 transcriptional repressors. None of the repressors passed the statistical significance threshold (P value < 0.01) due to small magnitudes of changes in gene expression and/or relatively low expression abundance. (e-g) Quantitative PCR analysis of a selection of inflammation-related transcripts using the samples that were sequenced. *P < 0.05 compared to wt