Fig. 2

Knockdown of MALAT1 attenuates neuroinflammation caused by LPS/ATP treatment in BV2 cells. After the BV2 cells were treated with LPS/ATP, LPS/ATP + sh-NC, and LPS/ATP + sh-MALAT1, a the expression of MALAT1 was analysed by qPCR. b The analysis of the cytokines TNF-α, IL-1β and IL-18 through ELISA for the different groups. c The mRNA levels of TNF-α, IL-1β and IL-18 were measured by qPCR in different groups. d The ROS levels in different groups were determined by fluorescence imaging of DCFH-DA. e The western blot analysis of cleaved caspase 1, NLRP3, ASC, Nrf2 and HO-1 levels in different groups. f The mRNA levels of HO-1, NQO-1, and SOD2 in different groups were measured by qPCR. The data in the graph are presented as the means ± SD as the relative levels from three replications. *p < 0.05, **p < 0.01, ***p < 0.001