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Fig. 3 | Molecular Brain

Fig. 3

From: LncRNA MALAT1 facilitates inflammasome activation via epigenetic suppression of Nrf2 in Parkinson’s disease

Fig. 3

MALAT1 binds EZH2 to recruit h3k27me3 and regulate the expression of Nrf2. After the BV2 cells were treated with LPS/ATP, LPS/ATP + sh-NC, and LPS/ATP + sh-MALAT1 (a) The expression of EZH2 and H3K27me3 was analysed by western blot. b The combination of MALAT1 and EZH2 analysed by RNA pull-down assay and EZH2 protein levels were detected by western blotting. Human antigen R (HuR) and ARE-mRNAs (AR) were used as negative control (c) RNA immunoprecipitation (RIP) experiments was performed in BV2 cells with an antibody EZH2, and the MALAT1 expression was detected by qPCR. d ChIP assay was used to investigate the NRF2 promoter expression after treating BV2 cells with shNC or shMALAT1. The data in the graph are presented as the means ± SD as the relative levels from three replications. *p < 0.05, **p < 0.01, ***p < 0.001

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