Fig. 3From: LncRNA MALAT1 facilitates inflammasome activation via epigenetic suppression of Nrf2 in Parkinson’s diseaseMALAT1 binds EZH2 to recruit h3k27me3 and regulate the expression of Nrf2. After the BV2 cells were treated with LPS/ATP, LPS/ATP + sh-NC, and LPS/ATP + sh-MALAT1 (a) The expression of EZH2 and H3K27me3 was analysed by western blot. b The combination of MALAT1 and EZH2 analysed by RNA pull-down assay and EZH2 protein levels were detected by western blotting. Human antigen R (HuR) and ARE-mRNAs (AR) were used as negative control (c) RNA immunoprecipitation (RIP) experiments was performed in BV2 cells with an antibody EZH2, and the MALAT1 expression was detected by qPCR. d ChIP assay was used to investigate the NRF2 promoter expression after treating BV2 cells with shNC or shMALAT1. The data in the graph are presented as the means ± SD as the relative levels from three replications. *p < 0.05, **p < 0.01, ***p < 0.001Back to article page