Fig. 4

MALAT1 activates the inflammasome through regulation of NRF2. BV2 cells were treated with LPS/ATP, LPS/ATP + sh-NC, LPS/ATP + sh-MALAT1, and LPS/ATP + sh-MALAT1 + ML385. a The analysis of the cytokines TNF-α, IL-1β and IL-18 through ELISA. b The mRNA levels of TNF-α, IL-1β and IL-18 in BV2 cells were analysed by qPCR. c The expression of inflammasome-related proteins, including NLRP3, ASC, cleaved caspase 1, NRF2, and HO-1 in BV2 cells were assessed by western blot analysis. d The mRNA levels of HO-1, NQO-1, and SOD2 in the BV2 cells were measured by qPCR. e The ROS levels in BV2 cells were assessed using DCFH-DA. f Measurements of antioxidants, including SOD and CAT activities, in BV2 cells by qPCR. The data in the graph are presented as the means ±SD as the relative levels from three replications. *p < 0.05, **p < 0.01