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Fig. 5 | Molecular Brain

Fig. 5

From: LncRNA MALAT1 facilitates inflammasome activation via epigenetic suppression of Nrf2 in Parkinson’s disease

Fig. 5

MALAT1 activates the inflammasome through regulation of EZH2. BV2 cells were treated with LPS/ATP, LPS/ATP + sh-NC, LPS/ATP + sh-MALAT1, and LPS/ATP + sh-MALAT1 + GSK-126. a The expression of MALAT1 after the BV2 cells were analysed by qPCR. b The analysis of the cytokines TNF-α, IL-1β and IL-18 through ELISA. c The mRNA levels of TNF-α, IL-1β and IL-18 were determined by qPCR. d The expression of inflammasome-related proteins, including NLRP3, ASC, cleaved caspase 1, NRF2, and HO-1 in BV2 cells were assessed by western blot analysis. e The mRNA levels of HO-1, NQO-1, and SOD2 in the BV2 cells as assessed by qPCR. f The ROS levels in BV2 cells were assessed using DCFH-DA. f Measurements of antioxidants, including SOD and CAT activities in BV2 cells. The data in the graph are presented as the means ±SD as the relative levels from three replications. *p < 0.05, **p < 0.01

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Molecular Brain

ISSN: 1756-6606