Fig. 4
From: mGluR5 regulates REST/NRSF signaling through N-cadherin/β-catenin complex in Huntington’s disease
mGluR5 modulates both REST/NRSF and SNAP-25 expression in primary neuronal cultures. Representative western blots and quantification of fold change in REST/NRSF and SNAP-25 protein expression with the corresponding loading controls in primary cultured corticostriatal neurons from E15 wild-type mouse embryos stimulated with either the mGluR group I agonist DHPG (100 μM) (a and b) or mGluR5-selective NAM, CTEP (10 μM) (c and d) for 10, 30, 60, 180 and 360 mins. REST/NRSF was normalized to vinculin, and SNAP-25 was normalized to actin (n = 3–4 for each group). Values represent mean ± SEM and are expressed as a fraction of the non-treated (NT) cultures. *P < 0.05 versus NT cultures. Statistical significance was assessed by one-way ANOVA and Fisher’s LSD multiple comparisons. REST/NSRF was probed on β-catenin blots and therefore the same vinculin blot is presented for REST/NRSF (Fig. 4a and c) and β-catenin (Fig. 1a and c)