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Table 1 Glutamine content is significantly diminished in FTD3 neurons derived from hiPSCs

From: Glutamate-glutamine homeostasis is perturbed in neurons and astrocytes derived from patient iPSC models of frontotemporal dementia

substrates present during incubation amino acid isogenic control neurons FTD 3 neurons P value  
amounts (nmol/mg protein)
[U- 13C]glucose Glutamine 12 ± 1.7 6 ± 1.3 0.005 a
Glutamate 61 ± 4.3 51 ± 2.9 0.14  
[U- 13C]glutamine + glucose Glutamine 18 ± 1.3 13 ± 1.5 0.008 a
Glutamate 68 ± 5.1 59 ± 4.0 0.26  
[U- 13C]glutamate + glucose Glutamine 14 ± 1.5 7 ± 0.7 0.024 a
Glutamate 185 ± 18.8 185 ± 18.8 1.00  
  1. Cellular content of amino acids in cultured neurons derived from hiPSCs. Cultured neurons were incubated for 90 min with 2.5 mM [U-13C] glucose or 0.25 mM [U-13C] glutamate or 0.5 mM [U-13C] glutamine in the presence of 2.5 mM unlabeled glucose. Cell extracts were analyzed by HPLC for determination of amino acid content. Results are mean ± SEM from 3 individuals (6 different cultures of hiPSCs-derived neurons/condition). Unpaired multiple t test followed by Bonferroni-Dunn post hoc test was used for statistical evaluations. P values were calculated comparing FTD3 neurons agains isogenic controls, a denotes statistical significance