substrates present during incubation
|
amino acid
|
isogenic control neurons
|
FTD 3 neurons
|
P value
| |
---|
amounts (nmol/mg protein)
|
[U- 13C]glucose
|
Glutamine
|
12 ± 1.7
|
6 ± 1.3
|
0.005
|
a
|
Glutamate
|
61 ± 4.3
|
51 ± 2.9
|
0.14
| |
[U- 13C]glutamine + glucose
|
Glutamine
|
18 ± 1.3
|
13 ± 1.5
|
0.008
|
a
|
Glutamate
|
68 ± 5.1
|
59 ± 4.0
|
0.26
| |
[U- 13C]glutamate + glucose
|
Glutamine
|
14 ± 1.5
|
7 ± 0.7
|
0.024
|
a
|
Glutamate
|
185 ± 18.8
|
185 ± 18.8
|
1.00
| |
- Cellular content of amino acids in cultured neurons derived from hiPSCs. Cultured neurons were incubated for 90 min with 2.5 mM [U-13C] glucose or 0.25 mM [U-13C] glutamate or 0.5 mM [U-13C] glutamine in the presence of 2.5 mM unlabeled glucose. Cell extracts were analyzed by HPLC for determination of amino acid content. Results are mean ± SEM from 3 individuals (6 different cultures of hiPSCs-derived neurons/condition). Unpaired multiple t test followed by Bonferroni-Dunn post hoc test was used for statistical evaluations. P values were calculated comparing FTD3 neurons agains isogenic controls, a denotes statistical significance