Fig. 2

Overexpression of Slit2 reduces spontaneous Ca²⁺ signal amplitude and frequency in the peri-infarct region. a, b Diagram showing the site of microinfarct (MI) induction and two-photon Ca²⁺ imaging. c Representative Ca²⁺ images in the peri-infarct area of a WT mouse. White circles show six representative neurons monitored for Ca²⁺ signals over 60 s. d Representative Ca²⁺ signals in the six representative neurons (regions of interest, ROIs) indicated in c. e Representative Ca²⁺ images of the peri-infarct area in a Slit2-Tg mouse. White circles show the six representative neurons monitored for 60 s. f Representative Ca²⁺ signals in the OGA-1 AM-positive neurons (ROIs) indicated in E. g Comparisons of Ca²⁺ signals between sham and MI groups of WT and Slit2-Tg mice. Each dataset is expressed as mean ± SD. ^*P ≤ 0.05; ^**P ≤ 0.01; ^***P ≤ 0.001; ^****P ≤ 0.0001. n = 6 mice