Fig. 1

Comparison of luciferase substrates for in vivo BLI using Bdnf-Luc mice. a. Structure of d-luciferin, seMpai, and TokeOni. b. Schedule of experiments. d-luciferin, seMpai, and TokeOni were administered intraperitoneally to Bdnf-Luc mice, and in vivo BLI was performed 5 min after each administration. Each substrate was injected into mice with at least 2 d intervals. c. Representative images of in vivo BLI using d-luciferin, seMpai, or TokeOni as a luciferase substrate. Bioluminescence; counts indicated by pseudocolored images. Photo; photographs corresponding to bioluminescence images. d. ROI analysis. Data represent the mean ± SEM of four independent experiments using one-way ANOVA with Tukey’s multiple comparisons test (*p < 0.05, **p < 0.01, and ****p < 0.0001). e. Line profiles [counts from (i) to (ii)]. Data represent the mean ± SEM of four independent experiments using two-way ANOVA with Dunnett’s multiple comparisons test [#1; significant difference between d-Luciferin versus TokeOni (p < 0.05), #2; significant difference between d-Luciferin versus seMpai (p < 0.05)]