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Fig. 3 | Molecular Brain

Fig. 3

From: Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord

Fig. 3

Colocalization of HCN4-immunoreactivity with PKCγ- or PV-immunoreactivity. a Colocalization of HCN4 and PKCγ. Left panel, immunohistochemistry for HCN4 (red). Center panel, PKCγ immunohistochemistry (white). Right panel, overlay of HCN4- and PKCγ-immunoreactivities. Arrowheads indicate cells IR for both HCN4 and PKCγ. b Colocalization of HCN4 and PV. Left panel, HCN4 immunohistochemistry (red). Center panel, immunohistochemistry for PV (cyan). Right panel, overlay of HCN4- and PV-immunoreactivities. Arrowheads indicate cells IR for both HCN4 and PV. Note that the PV-IR intensity in HCN4-IR cells is relatively weak. a, b Scale bars, 40 μm. Dashed lines indicate the borders of the PKCγ plexus. c, d Proportions of HCN4-IR cells in lamina IIiv (c) and III (d) that colocalize with PKCγ- and/or PV-immunoreactivities

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