Fig. 1
Characterization of presynaptic short-term plasticity and mitochondria in the mPFC of adult Cyfip2+/− mice. a Representative traces and quantification of eEPSCs (normalized to the first eEPSC amplitude) induced by a 10 Hz train of stimuli in the mPFC layer 5 neurons of wild-type (WT) and Cyfip2+/− (heterozygous, HET) mice. Numbers of cells and mice used for the experiments are indicated. b Representative traces and quantification of normalized eEPSCs induced by a 20 Hz train of stimuli. c Representative dendritic segments reconstructed from serial block-face scanning electron microscopy (SB-SEM) images show dendritic spines (gray), presynaptic boutons (blue), and presynaptic mitochondria (yellow) in the mPFC layer 5 neurons of WT and HET mice (left panel). Quantification of the mitochondria-containing bouton ratio, presynaptic bouton density, and presynaptic mitochondron volume (right panels, n = 12 and 13 dendritic segments from 4 WT and 4 HET mice, respectively). Scale bar, 2 μm. NS, not significant. d Representative electron microscopic image and reconstruction of axonal process mitochondria in the mPFC layer 5 of WT and HET mice (left panels). Quantification of the number (in 73.5 μm3) and volume of mitochondria (right panels, n = 16 frames from 4 mice per genotype). Scale bars, 2 μm. e Representative electron microscopic images of the cristae structures of presynaptic mitochondria in the mPFC layer 5 of WT and HET mice (left panels). Quantification of the mitochondrial cristae density (right panel, n = 30 mitochondria from 3 mice per genotype). Scale bars, 0.5 μm. f List of 23 mitochondrial proteins of the forebrain CYFIP2 interactome (left panel). Interaction network of 23 mitochondrial proteins (yellow nodes) in the CYFIP2 interactome. Other mitochondrial proteins (grey nodes) were also included to generate the network. Mt., mitochondria. g Representative western blot images showing detection of CYFIP2 in mitochondrial fraction from the mPFC homogenate of adult WT mice. Mitofusin 2 (MFN2), translocase of outer mitochondrial membrane 40 (TOMM40), and optic atrophy 1 (OPA1) are representative mitochondrial proteins. Histone H3 is a nuclear protein (negative control). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01. (unpaired two-tailed Student’s t-test)