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Fig. 6 | Molecular Brain

Fig. 6

From: Peptidome analysis of cerebrospinal fluid in neonates with hypoxic-ischemic brain damage

Fig. 6

Effects of HIBDAP pretreatment on cell pyroptosis under OGD. a Bioinformatics analysis of HIBDAP; b FITC-labelled TAT-HIBDAP could successfully enter PC12 cells and further into the nucleus; c After HIBDAP pretreatment and 6 h of OGD treatment, the cell survival rate was significantly increased in PC12 cells pretreated with 1 μM, 5 μM, 10 μM and 20 μM HIBDAP except that of 40 μM group was significantly decreased; d The concentrations including 1 μM, 5 μM and 10 μM of HIBDAP significantly reduced pyroptosis of PC12 cells, except 20 μM had no effect; e Transmission electron microscopic examination showed that aggregated nuclear chromatin (the red circle), fuzzy cell membrane boundary (the red arrowhead), swollen endoplasmic reticulum (yellow arrowheads) in the OGD group (OGD), which could be alleviated by HIBDAP pretreatment (5 μM + OGD); f Compared with the control group (NC), the mRNA and protein expressions of NLRP3, NLRP1, ASC, and Caspase-1 were significantly increased in the OGD group (OGD) whereas HIBDAP pretreatment (5 μM + OGD) significantly suppressed their expressions except NLRP1 (nP > 0.05; *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001)

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