Fig. 1

The effects of anti-α2AP neutralizing antibodies on adult hippocampal neurogenesis. a Representative images of immunostaining of BrdU and Ki67. Anti-α2AP neutralizing antibodies or control IgG were intracerebroventricularly injected in 12-week-old C57BL/6J mice 2 h after the first intraperitoneal injection of BrdU, and BrdU was administered at 24-h intervals for 7 days. Coronal brain slices were immunostained with antibodies. Arrow heads indicate Ki67⁺ cells, and arrows indicate examples of BrdU⁺/Ki67⁻ cells. Scale bar: 50 μm. b The numbers of BrdU⁺/Ki67⁻ cells in the DG were counted in a blinded manner. c Representative images of immunostaining of Dcx. Scale bar: 200 μm. d The numbers of Dcx⁺ cells in the DG were counted in a blinded manner. The values represent the means ± S.E. (control IgG: n = 4, α2AP Ab: n = 5). Statistical significance was evaluated using Student’s t-test. *P < 0.05