Fig. 3

The effects of the injection of α2AP on adult hippocampal neurogenesis. a Representative images of immunostaining of BrdU and Ki67. α2AP or saline was intracerebroventricularly injected in 12-week-old C57BL/6J mice, 2 h after the first intraperitoneal injection of BrdU, and BrdU was administered at 24-h intervals for 7 days. Coronal brain slices were immunostained with antibodies. Arrow heads indicate Ki67⁺ cells. Scale bar: 50 μm. b The numbers of BrdU⁺/Ki67⁻ cells in the DG were counted in a blinded manner. c Representative images of immunostaining of Dcx. Scale bar: 200 μm. d The numbers of Dcx⁺ cells in the DG were counted in a blinded manner. The values represent the mean ± S.E. (saline: n = 5, α2AP: n = 6). Statistical significance was evaluated using Student’s t-test. *P < 0.05