Fig. 1

Acute oculomotor learning induces a decrease in the intrinsic excitability of Purkinje cells in the cerebellum. a Experimental design. Mice were subjected to oculomotor training for 50 min by rotating an optokinetic screen displaying vertically aligned white and black stripes (top). After the learning, cerebellar slices were prepared (middle) and whole-cell patch-clamp was performed from the floccular PCs located at the H zone (bottom). The dashed line represents the PC layer. b (Left) representative traces of the screen, eye movements before (middle) and after (bottom) the learning. In the middle and bottom traces, gray and black lines represent raw and curve-fitted traces, respectively. (Right) Learning-induced increase in OKR gain. A grey circle represents an individual animal (6 mice). Bar graphs indicate the mean values of each time-point. c Representative spiking responses in response to depolarizing current injections (bottom) into PCs of the control (top) and learned groups (middle). d The mean firing rate significantly decreases in the learned group compared to that of the control group. e OKR learning increases the rheobase current. f AP threshold, g AP amplitude, h AHP amplitude, and i Input resistance are comparable between the groups. In (d–i), the control and learned groups are depicted in white and black, respectively. Data represents as mean ± SEM