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Fig. 4 | Molecular Brain

Fig. 4

From: AAV9-Retro mediates efficient transduction with axon terminal absorption and blood–brain barrier transportation

Fig. 4

Comparison of retrograde infection tropism and efficiency between rAAV9-Retro and rAAV2-Retro at different brain regions. AH The two viruses were injected into the brain area of CPu (A, E), which was reported to mainly receive input from the CTX, BLA and TH areas via rAAV2-Retro. Similar to rAAV2-Retro (BD), rAAV9-Retro effectively infected these brain regions (FH). I, J Through quantitative analysis, we found that the efficiency was equivalent between them (i, from CTX: 6495 ± 479.8 for AAV2-Retro, 7119 ± 507.6 for AAV9-Retro, P = 0.4219; from BLA: 737.3 ± 19.19 for AAV2-Retro, 871 ± 203.1 for AAV9-Retro, P = 0.5481; from TH: 1281 ± 250.0 for AAV2-Retro, 945.0 ± 256.6 for AAV9-Retro, P = 0.4010). Additionally, no statistical difference was found in the total number of neurons projected into the CPu (J, 8888 ± 672.8 for AAV2-Retro, 9366 ± 633.6 for AAV9-Retro; P = 0.6318). The data are shown as means ± SEM. Statistical significance of the differences was set at P < 0.05. Scale bars = 200 μm. CPu: caudate putamen (striatum); CTX: cerebral cortex; BLA: basolateral amygdalar nucleus; TH: thalamus

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