Fig. 3

Trafficking of exon 3-GFP, exon 5-GFP, exon 7-GFP, and exon 8-GFP transcripts in hippocampal neurons. a–d Densitometric analysis of in situ hybridization on apical dendrites of neurons transfected with exon 3-GFP (a), exon 5-GFP (b), exon 7-GFP (c) and exon 8-GFP (d) untreated (gray boxes) or treated with 10 mM KCl for 3 h (white boxes). Below each graph, representative straightened neurons labeled by in situ hybridization are reported. Lines in graphs represent the plot of the median gray value of GFP densitometric analysis untreated (solid line) and after KCl stimulation (dashed line). Scale bars: 10 μm. *p ≤ 0.05 exon-GFP ctrl respect to GFP control and exon-GFP KCl vs GFP KCl. ^#p ≤ 0.05 exon-GFP ctrl vs exon-GFP KCl