Table 2 Primers and PCR conditions for BDNF 5′UTR cloning

Exon I

AATTCTCGAGTAAAGCGGTAGCCGGCTGGTGCAGG

GTCTACCGGITTTGCTGTCCTGGAGACTCAGTGTC

Denaturation

98 °C

10 s

31 cycles

Annealing

57 °C

20 s

Extension

72 °C

40 s

Exon IIa

GATCCTCGAGGCTTTGGGAAAGCCATCCGCACGTGAC

GCCACCGGTCTGGATGAAGTACTACCACCTCGGAC

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

35 s

Exon llb

GATCCTCGAGGCTTTGGCAAAGCCATCCGCACGTGAC

GTTACCGOTOCAGCTTGCCAAGAGTCTATTCCAG

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

35 s

Exon llc

GATCCTCGAGGCTTTGGCAAAGCCATCCGCACGTGAC

GA/\ACCGGTCTTCTTTGCGGCTTACACCACCCCGGTGGCTAG

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

35 s

Exon III

AGGCTCGAGGCCCTCACGATTCTCGCTGGATAG

GCTAACCGGTCTGGGCTCAATGAAGCATCCAGCCC

Denaturation

98 °C

15 s

31 cycles

Annealing

55 °C

15 s

Extension

68 °C

20 s

Exon IV

AAITCTCGAGACCCACTTTCCCATTCACCGAGG

GACTACCGGTCAGTCACTACTTGTCAAAGTAAACATCAAGGC

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

20 s

Exon V

GACTCTCGAGAAACCATAACCCCGCACACTCTGTGTAGTTTCATTGTGTGTTCG

GACTACCGGTCTTCCCGCACCTTCCCGCACCACAGAGCTAGAAAAAGCGAACACAC

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

15 s

Exon VI

GACTCTCGAGCCAATCGAAGCTCAACCGAAGAGC

GATCACCGGTCTCAGGGTCCACACAAAGCTCTCGG

Denaturation

98 °C

10 s

31 cycles

Annealing

57.5 °C

20 s

Extension

72 °C

15 s

Exon VII

GACTCTCGAGCACTGTCACCTGCTTTCTAGGGAGTATTACC

GTACACCGGTCTCCCGGATGAAAGTCAAAACTTTCACTTCCTCTGGAGG

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

20 s

Exon VIII

GTCACTCGAGGTTATAGAGTTGGATGCAAGCGTAACCCG

GCATACCGGTGACACCATTTCAGCAATCGTTTGTTCAGCTCC

Denaturation

98 °C

10 s

31 cycles

Annealing

58 °C

20 s

Extension

72 °C

35 s