Fig. 4

IL-1β expression is reduced in Hv1^−/− mice after SCI. a Representative images showing colocalization of IL-1β (green) with Iba1 (red) in the white matter of WT and Hv1^−/− mice in sham, 1 d, 3 d and 7 d after SCI (Scale: 100 um). b Bar graph shows intensity of IL-1β staining in the sham and SCI groups in WT (gray) and Hv1^−/− (blue) mice, and percentage of IL-1β staining co-localizing with Iba1 (orange). c Representative images of IL-1β staining in ventral horn at various sagittal planes (R3-C3) along the spinal cord in WT and Hv1^−/− at 3 d after SCI (Scale: 100 um). d Bar graph denotes IL-1β staining intensity of R3-C3 in WT and Hv1^−/− at 3 d after SCI and is represented as mean ± SEM. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test)