Fig. 2

CCPs occasionally exist as multiples in close vicinity on somal/dendritic plasma membrane. The characteristic “coat” appearance was visible in samples treated with low concentrations of osmium tetroxide (0.2% in a, b), and labels for clathrin (a) and for AP2 (b) were specifically localized to the coat. The appearance of the coat was enhanced when tannic acid (1%) was added to fixative followed by regular osmium tetroxide (1% in c), and less conspicuous with “reduced osmium” treatment (1% potassium ferrocyanide + 1% osmium tetroxide in d). All samples were 3 week-old dissociated hippocampal cultures. Scale bar = 100 nm