Fig. 6

Depolarization induces redistribution of clathrin in presynaptic axon terminals of dissociated hippocampal cultures. Under control conditions, label for clathrin was concentrated outside of the SV clusters (a). Upon high K⁺ treatment, SVs became de-clustered, and label for clathrin became dispersed among the SVs (b). Distance measurements of label for clathrin showed a shift toward the presynaptic membrane upon high K⁺ treatment (c, representative histograms of data from exp 1 of Additional file 6)