Fig. 1

Strategy of the survey and validation of experimental groups. a Experimental setup. Differential expression of hippocampal RNA from untreated controls (cntrl, n = 4 replicates), from mice sacrificed at indicated time points after seizure onset (n = 3 replicates) and vehicle-treated time-matched controls (n = 3 replicates) was analyzed using exon microrarrays and exon usage was validated by RNA sequencing, in situ hybridization and RT-PCR. b Sample-wise correlation matrix of the 1000 genes with the highest standard deviation over all samples were extracted and used to construct a pairwise correlation matrix using Pearson correlation. c Principal component analysis of individual factor maps of significantly regulated genes. The two principal components PC1 and PC2 represent treatment and time after seizure onset, respectively. KA samples at indicated timepoints after kainic-acid induced seizure onset, veh. vehicle-treated time-matched controls