Fig. 5

NA facilitates inhibitory synaptic inputs onto SDH GRPR⁺ neurons. a A schematic illustration of patch-clamp recording of GRPR⁺ neurons in cervical spinal cord slices (parasagittally cut) from Grpr-EGFP mice. b Representative trace of spontaneous IPSCs in SDH GRPR⁺ neurons before and after application of NA (20 μM). The lower panels show the traces in expanded time scale. Quantitative analysis of the effects of NA (20 μM, n = 13 cells), the α₁-AR agonist phenylephrine (20 μM, n = 13 cells) and the α_1A-AR agonist A61603 (5 μM, n = 6 cells) on the frequency (c) and amplitude (d) of spontaneous IPSCs in GRPR⁺ SDH neurons. *P < 0.05, and **P < 0.01. Data show the mean ± SEM