Fig. 7

Contextual and cued fear memory of protrudin-deficient mice. a–j Percent freezing time (a–e) and distance traveled (f–j) during conditioning (a, f), in a context test performed 1 day (b, g) or 28 days (d, i) after conditioning, and in a cued test with altered context performed 2 days (c, h) or 29 days (e, j) after conditioning in the contextual and cued fear-conditioning test. Mice were presented three times with white noise as a conditioned stimulus (CS) for 30 s (horizontal black bars) followed by foot shock as an unconditioned stimulus (US) for the last 2 s of the conditioned stimulus (vertical arrows) during the conditioning session. k Shock sensitivity assessed on the basis of the distance traveled during and after exposure to the unconditioned stimulus. All data are means ± SEM (WT mice, n = 22; PRT^–/– mice, n = 17). P values for differences between genotypes were determined by two-way repeated-measures ANOVA (a–k). For the cued test, separate P values are shown for the first and second 3-min periods. The results of all statistical analysis are provided in Additional file 1: Table S1