Fig. 4

MeCP2 knockdown changes baseline and learning-associated splicing events. a Schematic representation of the comparisons used. b Proportion of each differential alternative splicing events (DAS) in baseline conditions (left) or in learning state (right) in MeCP2-shRNA conditions. c Number of inclusion (positive ΔPSI, blue) and exclusion (negative ΔPSI, red) events for each type of alternative splicing modality in MeCP2 knockdown during baseline and learning state conditions (q-value < 0.05). d Pie charts showing the proportion of inclusion and exclusion events for intron retention (IR) and exon skipping (ES) in baseline and learning state conditions. e Scatter plots showing changes in IR and ES events in home-cage (Baseline) and learning state (learning) conditions by MeCP2 knockdown. Red dots and blue squares represent alternative splicing events occurred in either baseline or learning state (q-value < 0.05) conditions, respectively. Green triangles represent alternative splicing events that occurred in both conditions (q-value < 0.05). f Violin plots showing the ΔPSI distribution of IR (left) and ES (right) events in baseline and learning state in the hippocampi of MeCP2-shRNA mice. The P-values are based on paired two-tailed Student’s t test or Wilcoxon test and are indicated at the top of each panel