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Fig. 1 | Molecular Brain

Fig. 1

From: WNT signaling suppresses oligodendrogenesis via Ngn2-dependent direct inhibition of Olig2 expression

Fig. 1

WNT signaling inhibit Olig2 expression through upregulation of Ngn2 expression. a Transverse sections of spinal cord at E12.5 from control and WNT signaling activated (Olig1Cre/+;Ctnnb1ΔEx3/+) mice were subjected to IF with anti-OLIG2 antibody or ISH with Olig1, Pdgfrα and Ngn2 riboprobes. The cells positive for OLIG2, Olig1 and Pdgfrα are absent in the spinal cord from Olig1Cre/+;Ctnnb1ΔEx3/+ mice, whereas Ngn2 is upregulated. Inset highlights the expression of OLIG2 in pMN domain, note that vascular development was abnormal in the spinal cord of Olig1Cre/+;Ctnnb1ΔEx3/+ mice. b There are putative Ngn2 binding sequences in the promoter regions of Ngn2 from human, rat and mouse. c Luciferase report assay revealed that Ngn2 but not its DNA binding deficient mutant AQ-Ngn2 inhibit the promoter activity of mouse Olig2. *p < 0.05, t-test. d Over-expression of Ngn2-EnR mimics the phenotype caused by over-expression of Ctnnb1-ΔEx3. Both expression of Ctnnb1-ΔEx3 and Ngn2-EnR suppressed the expression of OLIG2 in ovo. Arrowhead indicates induced expression of chick Ngn2 (cNgn2). Arrows represent reduced expression of endogenous genes. e OLIG2 maintains proliferation of pMN domain neural progenitor cells. High level of WNT signaling upregulates Ngn2 expression, NGN2 in turn coordinate with OLIG2 to promote motor neurons specification and suppress Olig2 expression in newly generated motor neurons. OPCs were specified OLIG2+ cells when WNT signaling is declined at the gliogenesis stage

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