Fig. 2

CCR2 is widely expressed in DRG neurons and displays upregulation following peripheral inflammation. a, b Typical examples and quantitative summary from dual immunofluorescence experiments showing CCR2 immunoreactivity IB4-binding non-peptidergic DRG neurons in the naïve and CFA-inflamed mice. c, d Immunofluorescence experiments and quantitative summary showing the coexistence of CCR2 immunoreactivity in CGRP-expressing peptidergic DRG neurons in naïve mice and its upregulation in CFA-inflamed mice. e, f Immunofluorescence staining and quantitative summary showing the colocalization of CCR2 immunoreactivity with NF200-positive large-diameter DRG neurons in naïve mice and no significant change in expression level after CFA inflammation. Note that in CFA-inflamed group, ipsilateral L3/L4 DRGs were obtained and analyzed at 24 h after intraplantar CFA injection. Arrows indicate the co-expressing cells. **P < 0.01. Two-Way ANOVA followed by post-hoc Bonferroni test (n = 4 mice/group). Data are expressed as mean ± SEM