Fig. 1
From: Altered striatal dopamine levels in Parkinson’s disease VPS35 D620N mutant transgenic aged mice
Generation of VPS35 D620N Tg mice. a Construct design. The construct designed for VPS35 D620N mouse generation is inserted into the mouse Rosa26 locus under the control of an exogenous CAGGS promoter. A transcriptional termination signal sequence (flanked by two loxP sites) is present between the promoter and transgene coding sequence. Therefore, Cre recombinase is required to derepress the transgene via the excision of the transcriptional termination signal sequence. HA sequence was inserted. b PCR testing for VPS35 D620N. VPS35 D620N Tg mice and the positive control display a band at 525 bp, while NTg mice and the negative control do not. All mice are Nestin cre positive and display a band at 200 bp