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Fig. 7 | Molecular Brain

Fig. 7

From: Altered striatal dopamine levels in Parkinson’s disease VPS35 D620N mutant transgenic aged mice

Fig. 7

ARG evaluation of [3H]FE-PE2I binding to DAT in the striatum. a DAT binding in striatum. [3H]FE-PE2I binds to DAT with high potency. This binding is inhibited by the DAT inhibitor GBR12909. Thus, in vitro ARG using radiolabeled analogues of PE2I provides detailed qualitative evidence of the binding to DAT. Here, ARG is performed on striatal sections of 22-months-old NTg and Tg mice. Images show total binding of the radiolabeled tracer [3H]FE-PE2I (upper panel), and nonspecific binding in adjacent brain sections that were co-incubated in the presence of GBR12909 (lower panel). b Quantitative analysis of the radioactivity level in the striatum of DAT binding (pmol/g) between NTg and Tg mice (mean ± SEM). There was no significant difference (P = 0.216) in striatal DAT binding between NTg and Tg mice. n = 3. Student’s t-test

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