Fig. 5

LRP4 LDLα domain in astrocytes promotes dendrite arborization in primary co-cultured neurons. a Schematic diagram of time course dealing with co-culture neurons with astrocytes. b Representative images of neurons staining in co-culture. Primary neurons of mr-Lrp4^mitt mice or wild-type mice were co-cultured with astrocytes from mr-Lrp4^mitt mice or wild-type mice; Using the ImageJ plugin tracing the neurite branches; c Co-cultured with astrocytes from mr-Lrp4^mitt mice, neuronal dendrite branch number of wild-type mice was more than co-cultured with astrocytes from wild-type mice. A similar difference is present in the co-cultured neurons of mr-Lrp4^mitt mice with astrocytes from mr-Lrp4^mitt mice or wild-type mice. Using the ImageJ plugin for Sholl analysis. (Scale bar = 30 μm; Wild-type mice, n = 7; mr-Lrp4^mitt mice, n = 8; Neuron per group, n = 52. Values are means ± SEM. Multiple comparisons with two-way ANOVA analyses were also made between the groups. * P < 0.05, ** P < 0.01, *** P < 0.001)