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Fig. 1 | Molecular Brain

Fig. 1

From: Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice

Fig. 1

Doxycycline (Dox)-regulated Gad1 suppression system. a Generation of Gad1STOP−tetO knock-in mice. Schematic diagram depicting Gad1 genomic DNA (Gad1+), targeting vector, and Gad1 genomic DNA inserted into the Neo-STOP-tetO cassette (Gad1STOP−tetO). Arrows indicate the PCR primers (Primer set 1). b Schematic representation of Gad1 genomic DNA (Gad1+) and DNA containing the tetracycline-controlled transactivator (tTA) gene (Gad1tTA). Arrows indicate the PCR primers (Primer set 2). c, d Schematic diagram of the Dox-regulated Gad1 suppression system in Gad1tTA/STOP−tetO mice. Before Dox treatment, tTA binds to the tetracycline operator site (tetO) and promotes Gad1 transcription and GAD67 production (c). Dox treatment interferes with tTA binding to tetO and suppresses Gad1 transcription (d). e, f PCR genotyping. Representative results of PCR genotyping for Gad1tTA/STOP−tetO mice (e), Gad1STOP−tetO/STOP−tetO mice (f) and their littermates. M; 100-bp size marker, +/+; Gad1+/+, tTA/tet; Gad1tTA/STOP−tetO, tTA/ + ; Gad1tTA/+, tet/ + ; Gad1STOP−tetO/+, tet/tet; Gad1STOP−tetO/STOP−tetO

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