Fig. 2
From: Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice
Characterization of homozygous Gad1STOP−tetO/STOP−tetO mice. a The number of mouse pups born from heterozygous Gad1STOP−tetO/+ parents. +/+; Gad1+/+, tet/+; Gad1STOP−tetO/+, tet/tet; Gad1STOP−tetO/STOP−tetO. b The survival rates of mouse pups born from heterozygous Gad1STOP−tetO/+ parents. c Microscopic images of the palate of mice. The arrow indicates a cleft palate (CP). d Representative western blot analysis of the expression of GAD65/67 and β-actin proteins in the brains of Gad1+/+, Gad1STOP−tetO/+ and Gad1STOP−tetO/STOP−tetO mice. e Western blot analysis of the expression of GAD65/67 and β-actin proteins in the brains of 3 Gad1STOP−tetO/STOP−tetO mice with and without CP. The Gad1+/+ mouse protein sample was used as a positive control for GAD67 immunoblotting