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Fig. 3 | Molecular Brain

Fig. 3

From: Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice

Fig. 3

Characterization of Gad1tTA/STOP−tetO mice in the absence of Dox treatment. a Microscopic images of the palates of mice. b The survival rates of Gad1+/+ (+/+), Gad1tTA/STOP−tetO (tTA/tet), Gad1tTA/+ (tTA/+) and Gad1STOP−tetO/+ (tet/+) mice. The number of mice at postnatal day 0 was 15 (+/+), 20 (tTA/tet), 15 (tTA/+) and 17 (tet/+). c The number of mice with four genotypes at postnatal days 21–28. df Representative western blot analysis of the expression of GAD65/67 and β-actin proteins in the frontal cortex (d), the hippocampus (e) and the cerebellum (f) of Gad1+/+, Gad1tTA/STOP−tetO, Gad1tTA/+ and Gad1STOP−tetO/+ mice. β-Actin was used as a loading control. Three independent mice of the respective genotypes were examined. The band densities of GAD67 and β-actin were quantified by ImageJ software. GAD67 protein levels were normalized to β-actin protein expression, and the % change was calculated relative to Gad1+/+ mice. The means with SE are demonstrated as columns. *p < 0.05 vs. the value of Gad1+/+ mice (Bonferroni test)

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