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Table 1 Q-PCR assay was conducted to quantify the egfp expression cassette copy number in H1 or H8 infected cells in vitroa

From: High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy

Sample

UL37 quantity

UL37 adjusted quantity

egfp quantity

Adjusted relative ratio

Average ratio

H1

21,996,416.00

23,379,909.17

18,561,812.00

0.79

1.00 ± 0.11

H1

21,587,338.00

22,945,101.68

23,500,664.00

1.02

H1

20,390,316.00

21,672,791.42

25,614,332.00

1.18

H8

14,824,173.00

15,756,558.63

37,436,844.00

2.38

2.30 ± 0.06b

H8

13,998,001.00

14,878,423.46

34,695,536.00

2.33

H8

14,782,463.00

15,712,225.22

34,447,996.00

2.19

  1. a BHK cells were infected with H1 or H8 virus at MOI = 0.1 in a six-well plate. After 48 h, the supernatant was discarded and the cells were rinsed with PBS before scraped down for DNA or protein extraction. Gene copy number of GOI were determined by qPCR assay with the iQ SYBR Green Supermix kit (Bio-Rad). Results were expressed as means ± SEM of each group
  2. b Significant difference (p < 0.001), compared with H1 group