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Fig. 1 | Molecular Brain

Fig. 1

From: A new mouse line with reduced GluA2 Q/R site RNA editing exhibits loss of dendritic spines, hippocampal CA1-neuron loss, learning and memory impairments and NMDA receptor-independent seizure vulnerability

Fig. 1

Generation of GluA2+/ECS(G) mice and GluA2 Q/R site editing efficiency analysis. a Schematic representation of the i) GluA2 WT allele, ii) targeted GluA2+/ECS(G)neo allele and iii) the targeted GluA2+/ECS(G) allele, after the removal of the floxed neo cassette by Cre-mediated recombination. Exons 10, 11 and 12 are shown (black boxes). Black arrows indicate loxP sites. The position of the cytosine to guanine mutation within the ECS is indicated in red. White arrows indicate primer sets used for genotype analysis. b DNA sequencing of WT and GluA2+/ECS(G) mice confirmed the single cytosine to guanine mutation in the ECS of heterozygous mice, as highlighted in yellow. c Genotype analysis of WT and GluA2+/ECS(G) mice by PCR shows a band at 200 bp in WT and two bands at 200 bp and 250 bp in heterozygous mice. d GluA2+/ECS(G) mice exhibit a significant increase in the proportion of unedited GluA2(Q) (n = 5/genotype; Mann-Whitney t-test). e Representative image of sequences from WT and GluA2+/ECS(G) mice. The red arrow indicates the increased presence of A nucleotide indicating unedited RNA at the Q/R site of GluA2

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