Fig. 4
From: Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons
Serial sections (1–17 in center column) through two axons (yellow and blue) containing synaptophysin-labeled aggregates (marked as a, b & c) of vesicles/vacuoles at 5 DIV. Insets on both sides of the serial sections show enlargements of these aggregates at various sections. The axon segments containing aggregates a & c do not have contact with dendrites, whereas aggregate d in sections 16–17 is in contact with a dendrite (pink), perhaps forming a nascent synapse. Thick arrows in insets a5 & b5 point to clathrin-coated vesicles