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Table 1 Labeling densities (number of particles per μm2) of different SV proteins at the Golgi complex vs. cytoplasm in neuronal soma, and at clusters of SV-like vesicles in axon

From: Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons

 antibodysomaaxon
GolgicytoplasmRatio G/Cclusters of SV-like vesicles
SV membrane proteinssynaptophysinExp 116.4 ± 1.4 (6)4.5 ± 0.5 (8)3.64**395.5 ± 26.6 (13)
Exp 211.1 ± 1.3 (6)3.0 ± 0.4 (8)3.70*301.3 ± 25.1 (12)
SV2Exp 127.6 ± 3 .6 (5)6.5 ± 0.8 (7)4.25*262.6 ± 30.9 (10)
Exp 216.9 ± 1.7 (5)4.7 ± 0.5 (5)3.60*411.6 ± 35.2 (11)
SV-associated proteinsSynapsin IExp 11.7 ± 0.1 (10)4.8 ± 0.6 (12)0.35**158.4 ± 24.6 (8)
Exp 23.4 ± 0.3 (5)8.6 ± 0.9 (8)0.40**244.1 ± 27.0 (11)
α-synucleinExp 14.0 ± 0.6 (5)10.0 ± 1.0 (5)0.40*115.4 ± 13.2 (7)
Exp 22.2 ± 0.4 (3)6.7 ± 2.6 (3)0.33 NS181.3 ± 25.1 (6)
  1. (n) = number of Golgi, neuronal somal cytoplasmic area, and clusters of SV-like vesicles sampled
  2. Ratio G/C – labeling density of Golgi divided by that of cytoplasm. Difference in labeling density tested by Student t-test: * P < 0.005, **P < 0.0005, NS – not significant