From: Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons
antibody | soma | axon | ||||
---|---|---|---|---|---|---|
Golgi | cytoplasm | Ratio G/C | clusters of SV-like vesicles | |||
SV membrane proteins | synaptophysin | Exp 1 | 16.4 ± 1.4 (6) | 4.5 ± 0.5 (8) | 3.64** | 395.5 ± 26.6 (13) |
Exp 2 | 11.1 ± 1.3 (6) | 3.0 ± 0.4 (8) | 3.70* | 301.3 ± 25.1 (12) | ||
SV2 | Exp 1 | 27.6 ± 3 .6 (5) | 6.5 ± 0.8 (7) | 4.25* | 262.6 ± 30.9 (10) | |
Exp 2 | 16.9 ± 1.7 (5) | 4.7 ± 0.5 (5) | 3.60* | 411.6 ± 35.2 (11) | ||
SV-associated proteins | Synapsin I | Exp 1 | 1.7 ± 0.1 (10) | 4.8 ± 0.6 (12) | 0.35** | 158.4 ± 24.6 (8) |
Exp 2 | 3.4 ± 0.3 (5) | 8.6 ± 0.9 (8) | 0.40** | 244.1 ± 27.0 (11) | ||
α-synuclein | Exp 1 | 4.0 ± 0.6 (5) | 10.0 ± 1.0 (5) | 0.40* | 115.4 ± 13.2 (7) | |
Exp 2 | 2.2 ± 0.4 (3) | 6.7 ± 2.6 (3) | 0.33 NS | 181.3 ± 25.1 (6) |