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Table 1 Labeling densities (number of particles per μm2) of different SV proteins at the Golgi complex vs. cytoplasm in neuronal soma, and at clusters of SV-like vesicles in axon

From: Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons

 

antibody

soma

axon

Golgi

cytoplasm

Ratio G/C

clusters of SV-like vesicles

SV membrane proteins

synaptophysin

Exp 1

16.4 ± 1.4 (6)

4.5 ± 0.5 (8)

3.64**

395.5 ± 26.6 (13)

Exp 2

11.1 ± 1.3 (6)

3.0 ± 0.4 (8)

3.70*

301.3 ± 25.1 (12)

SV2

Exp 1

27.6 ± 3 .6 (5)

6.5 ± 0.8 (7)

4.25*

262.6 ± 30.9 (10)

Exp 2

16.9 ± 1.7 (5)

4.7 ± 0.5 (5)

3.60*

411.6 ± 35.2 (11)

SV-associated proteins

Synapsin I

Exp 1

1.7 ± 0.1 (10)

4.8 ± 0.6 (12)

0.35**

158.4 ± 24.6 (8)

Exp 2

3.4 ± 0.3 (5)

8.6 ± 0.9 (8)

0.40**

244.1 ± 27.0 (11)

α-synuclein

Exp 1

4.0 ± 0.6 (5)

10.0 ± 1.0 (5)

0.40*

115.4 ± 13.2 (7)

Exp 2

2.2 ± 0.4 (3)

6.7 ± 2.6 (3)

0.33 NS

181.3 ± 25.1 (6)

  1. (n) = number of Golgi, neuronal somal cytoplasmic area, and clusters of SV-like vesicles sampled
  2. Ratio G/C – labeling density of Golgi divided by that of cytoplasm. Difference in labeling density tested by Student t-test: * P < 0.005, **P < 0.0005, NS – not significant