Fig. 1

Slow EPSCs are mediated by TRP channels and independent of PLC in O/A INs, but absent in stratum radiatum interneurons. a. Left: representative traces of slow EPSCs elicited by HFS protocol in CA1 O/A INs of acute slices in control condition (black trace) and in presence of the TRP channel blocker SKF96365 (30 μM SKF, gray trace). Right: Summary bar graphs of peak EPSC amplitude for all cells in each condition. b. Left: representative slow EPSCs in O/A INs in acute slices in absence (black trace) and presence of PLC inhibitor (10 μM U73122, gray trace). Right: Summary bar graphs of peak EPSC amplitude for all cells in each condition. c. Left: Representative traces showing synaptic currents elicited by HFS in cultured hippocampal slices. Slow EPSCs were elicited in O/A INs (top), but were not elicited in stratum radiatum interneurons (bottom). Right: Summary bar graphs of peak EPSC amplitude for all cells in each condition. Numbers above bar graphs represent the number of cells in each group; *p < 0.05. Scale bars: 250 ms, 100 pA